{"id":4495,"date":"2017-04-18T12:37:11","date_gmt":"2017-04-18T10:37:11","guid":{"rendered":"https:\/\/gehrke-media.de\/gobio-2025\/mikrokerntest\/"},"modified":"2017-04-18T12:37:11","modified_gmt":"2017-04-18T10:37:11","slug":"mikrokerntest","status":"publish","type":"page","link":"https:\/\/gobio-gmbh.de\/en\/mikrokerntest\/","title":{"rendered":"Applied Cell Biology"},"content":{"rendered":"
[vc_custom_heading text=”Healthy Stem Cell Services” use_theme_fonts=”yes”]

In vitro assessment of the effects on the vertebrate genome is currently based on conventional, destructive nuclear staining procedures applied to immortalized mammalian cell lines (OECD TG 487). The use of our self-signaling cell line KCBGFP<\/span> (DSMC ACC3285) has an excellent potential to replace the previously used lines. Our technique to visualize nuclear structures based on inherited green fluorescent labeling of functional chromosomes reduces the cost of the method on the order of more than 80%.<\/p>\n<\/div><\/div>

<\/div>[vc_custom_heading text=”Cell coated plate technology” use_theme_fonts=”yes”]

This new technology enables providing ready-to-use cell coated 96-well culture plates.
\nAfter a short pre-culture period (12-24 hrs) under ambient temperature and CO2 conditions the cells grow to a 60% confluent monolayer and are then ready for exposure.<\/p>\n<\/div><\/div>

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Self-signaling nuclear structures of healthy cells (a-b)<\/em>
\nLoss of chromosomes caused by genotoxic compounds (c-d)<\/em><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>

[vc_custom_heading text=”Exposure to Test compound” use_theme_fonts=”yes”]
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Effects on nuclear structures of living cells\u00a0 can be recorded\u00a0 after 12-14 h exposure to the test compound and a recovery period of 2-24 hours\u00a0 by inverse fluorescence microcopy.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/section>

[vc_custom_heading text=”Genotoxicity, Cytotoxicity and Cytostatic Testing” use_theme_fonts=”yes”]