{"id":4437,"date":"2017-04-18T12:52:41","date_gmt":"2017-04-18T10:52:41","guid":{"rendered":"https:\/\/gehrke-media.de\/gobio-2025\/umu-test\/"},"modified":"2025-09-22T08:45:30","modified_gmt":"2025-09-22T06:45:30","slug":"umu-test","status":"publish","type":"page","link":"https:\/\/gobio-gmbh.de\/en\/umu-test\/","title":{"rendered":"umu test"},"content":{"rendered":"<section class=\"l-section wpb_row height_large\"><div class=\"l-section-h i-cf\"><div class=\"g-cols vc_row via_grid cols_1 laptops-cols_inherit tablets-cols_inherit mobiles-cols_1 valign_top type_default stacking_default\"><div class=\"wpb_column vc_column_container\"><div class=\"vc_column-inner\"><div class=\"g-cols wpb_row via_grid cols_2 laptops-cols_inherit tablets-cols_inherit mobiles-cols_1 valign_top type_default stacking_default\" style=\"--columns-gap:3rem;\"><div class=\"wpb_column vc_column_container\"><div class=\"vc_column-inner\"><div class=\"wpb_text_column\"><div class=\"wpb_wrapper\"><p>The umu test according to DIN EN ISO 38415-T3 1996-12 determines the mutagenic potential of water.<\/p>\n<p>The genetically modified bacterium <em>Salmonella typhimurium TA 1535\/pSK 1002<\/em>, a gram-negative, facultatively anaerobic bacterium from the Enterobacteriaceae family, serves as the test organism. This strain has some important properties for a sensitive genotoxicity test:<\/p>\n<ul>\n<li>The bacterium used carries a modified genome. It is excision repair deficient (uvrB), has an incomplete cell wall (rfa) that is permeable to many substances, and no chromosomal lacZ gene.<\/li>\n<li>Compared to the original strain, the test organism still carries the plasmid pSK1002 with the umuC-lacZ gene and a gene for ampicillin resistance.<\/li>\n<\/ul>\n<\/div><\/div><\/div><\/div><div class=\"wpb_column vc_column_container\"><div class=\"vc_column-inner\"><div class=\"w-image align_none\"><div class=\"w-image-h\"><img decoding=\"async\" width=\"900\" height=\"675\" src=\"https:\/\/gobio-gmbh.de\/wp-content\/uploads\/2025\/06\/umu-gobio-003.jpg\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/gobio-gmbh.de\/wp-content\/uploads\/2025\/06\/umu-gobio-003.jpg 900w, https:\/\/gobio-gmbh.de\/wp-content\/uploads\/2025\/06\/umu-gobio-003-300x225.jpg 300w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/div><\/div><\/div><\/div><\/div><div class=\"w-separator size_medium\"><\/div><div class=\"g-cols wpb_row via_grid cols_2 laptops-cols_inherit tablets-cols_inherit mobiles-cols_1 valign_top type_default stacking_default\" style=\"--columns-gap:3rem;\"><div class=\"wpb_column vc_column_container\"><div class=\"vc_column-inner\"><div class=\"w-image align_none\"><div class=\"w-image-h\"><img decoding=\"async\" width=\"1000\" height=\"750\" src=\"https:\/\/gobio-gmbh.de\/wp-content\/uploads\/2025\/06\/gobio-umu-test-bsp-001.jpg\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/gobio-gmbh.de\/wp-content\/uploads\/2025\/06\/gobio-umu-test-bsp-001.jpg 1000w, https:\/\/gobio-gmbh.de\/wp-content\/uploads\/2025\/06\/gobio-umu-test-bsp-001-300x225.jpg 300w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><\/div><\/div><\/div><\/div><div class=\"wpb_column vc_column_container\"><div class=\"vc_column-inner\"><div class=\"wpb_text_column\"><div class=\"wpb_wrapper\"><p>The bacteria are exposed to different concentrations of the test material under defined conditions. Depending on their concentration, genotoxins induce the so-called umuC gene. The induction of the gene is a measure of the mutagenic potential of the test material under investigation. Since the umuC gene in the test organism used is linked to the lacZ gene for \u00df-galactosidase, it is possible to measure the induction rate of the umuC gene by determining the galactosidase activity.<\/p>\n<p>Therefore, the method is only applicable to test materials where intrinsic \u00df-galactosidase activity is excluded. False positive results are to be expected, for example, with biotechnological process wastewater containing galactosidase-positive test organisms.<\/p>\n<p>The umu test is used to detect genotoxic effects as part of wastewater monitoring.<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>\n","protected":false},"excerpt":{"rendered":"The umu test according to DIN EN ISO 38415-T3 1996-12 determines the mutagenic potential of water. The genetically modified bacterium Salmonella typhimurium TA 1535\/pSK 1002, a gram-negative, facultatively anaerobic bacterium from the Enterobacteriaceae family, serves as the test organism. This strain has some important properties for a sensitive genotoxicity test: The bacterium used carries a...","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4437","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/pages\/4437","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/comments?post=4437"}],"version-history":[{"count":1,"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/pages\/4437\/revisions"}],"predecessor-version":[{"id":4440,"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/pages\/4437\/revisions\/4440"}],"wp:attachment":[{"href":"https:\/\/gobio-gmbh.de\/en\/wp-json\/wp\/v2\/media?parent=4437"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}